raft-generated polyacrylamide-dna block copolymers

RAFT-Generated Polyacrylamide-DNA Block Copolymers

Capillary electrophoretic separation of a mixture of 5′-fluorescein isothiocyanate-labeled single-stranded DNA (normal ssDNA) and its single-base-substituted one (mutant ssDNA) was achieved by using a RAFT-generated polyacrylamide-oligodeoxyribonucleotide block copolymer (PAAm-b-ODN) as an affinity polymeric probe.

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raft-generated polyacrylamide-dna block copolymers

RAFT-Generated Polyacrylamide-DNA Block Copolymers

RAFT-Generated Polyacrylamide-DNA Block Copolymers for Single-Nucleotide Polymorphism Genotyping by Affinity Capillary Electrophoresis - Biomacromolecules (ACS Publications)

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raft-generated polyacrylamide-dna block copolymers

RAFT-generated polyacrylamide-DNA block copolymers

Abstract Capillary electrophoretic separation of a mixture of 5'-fluorescein isothiocyanate-labeled single-stranded DNA (normal ssDNA) and its single-base-substituted one (mutant ssDNA) was achieved by using a RAFT-generated polyacrylamide-oligodeoxyribonucleotide block copolymer (PAAm-b-ODN) as an affinity polymeric probe.

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raft-generated polyacrylamide-dna block copolymers

RAFT-Generated Polyacrylamide-DNA Block Copolymers

Capillary electrophoretic separation of a mixture of 5'-fluorescein isothiocyanate-labeled single-stranded DNA (normal ssDNA) and its single-base-substituted one (mutant ssDNA) was achieved by...

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raft-generated poly(n-isopropylacrylamide)–dna block

RAFT-generated poly(N-isopropylacrylamide)–DNA block

A diblock copolymer containing a single-stranded DNA and a temperature-responsive poly(N-isopropylacrylamide) (PNIPAAm-b-DNA) was synthesized.The PNIPAAm segment was obtained with a low polydispersity by reversible addition–fragmentation chain transfer polymerization.

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raft-generated poly(n-isopropylacrylamide)-dna block

RAFT-generated poly(N-isopropylacrylamide)-DNA block

A diblock copolymer containing a single-stranded DNA and a temperature-responsive poly (N-isopropylacrylamide) (PNIPAAm-b-DNA) was synthesized. The PNIPAAm segment was obtained with a low polydispersity by reversible addition-fragmentation chain transfer polymerization.

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raft-generated poly(n-isopropylacrylamide)–dna block

RAFT-generated poly(N-isopropylacrylamide)–DNA block

A diblock copolymer containing a single-stranded DNA and a temperature-responsive poly (N -isopropylacrylamide) (PNIPAAm- b -DNA) was synthesized. The PNIPAAm segment was obtained with a low polydispersity by reversible addition–fragmentation chain transfer polymerization.

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water-soluble polymers. 81. direct synthesis

Water-Soluble Polymers. 81. Direct Synthesis

RAFT-Generated Polyacrylamide-DNA Block Copolymers for Single-Nucleotide Polymorphism Genotyping by Affinity Capillary Electrophoresis. Biomacromolecules 2009, 10 (4) , 805-813. DOI: 10.1021/bm801301b.

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raft-generated poly(n-isopropylacrylamide)–dna block

RAFT-generated poly(N-isopropylacrylamide)–DNA block

A diblock copolymer containing a single-stranded DNA and a temperature-responsive poly(N-isopropylacrylamide) (PNIPAAm-b-DNA) was synthesized.The PNIPAAm segment was obtained with a low polydispersity by reversible addition–fragmentation chain transfer polymerization.

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functional raft polymers for biomedical applications

Functional RAFT Polymers for Biomedical Applications

To date, efforts have focused on the use of RAFT polymerization for generating block copolymer micelles, vesicles, star polymers, nanoparticles, and capsules as potential advanced drug carriers and also polymer-drug conjugates as prodrugs (Figure 1). 1 This review focuses on the overview of the RAFT process, selection of appropriate RAFT agents

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dna terminal mismatch-induced stabilization of polymer

DNA Terminal Mismatch-Induced Stabilization of Polymer

DNA Terminal Mismatch-Induced Stabilization of Polymer Micelles from RAFT-Generated Poly(N-isopropylacrylamide)-DNA Block Copolymers Article in Chemistry - An Asian Journal 8(12) · December 2013

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www2.riken.jp

www2.riken.jp

RAFT-generated Polyacrylamide-DNA Block Copolymers for Single-nucleotide Polymorphism Genotyping by Affinity Capillary Electrophoresis; Naoki Kanayama, Hideaki Shibata, Ayumi Kimura, Daisuke Miyamoto, Tohru Takarada, Mizuo Maeda, Biomacromolecules, 10, 805-813, (2009). 80. 2009 (3)

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synthesis and characterization of the block copolymers

Synthesis and characterization of the block copolymers

The multi instruments studies of the obtained block copolymers show that the copolymers easily formed as a result of RAFT. Mn,GPC values of the copolymers were between 24,900 g.mol−1 and 74,100 g.mol−1.

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dna terminal mismatch‐induced stabilization of polymer

DNA Terminal Mismatch‐Induced Stabilization of Polymer

Temperature‐responsive diblock copolymers made of poly(N‐isopropylacrylamide) (PNIPAAm) generated by reversible addition‐fragmentation chain transfer (RAFT) polymerization and a single‐stranded DNA (ssDNA) self‐assembled into polymer micelles.The micelles consisted of the PNIPAAm core surrounded by the ssDNA corona with a hydrodynamic diameter up to 300 nm in an aqueous medium above

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synthesis of dna conjugate by mechanochemical solid-state

Synthesis of DNA conjugate by mechanochemical solid-state

RAFT-generated polyacrylamide-DNA block copolymers for single-nucleotide polymorphism genotyping by affinity capillary electrophoresis. Kanayama N, Shibata H, Kimura A, Miyamoto D, Takarada T, Maeda M. Biomacromolecules, 10(4):805-813, 01 Apr 2009 Cited by: 6 articles | PMID: 19249847

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quantitative single-nucleotide polymorphism analysis

Quantitative single-nucleotide polymorphism analysis

Single-nucleotide polymorphisms (SNPs) 2 are highly abundant genetic variations resulting from single-base substitution found in various organisms. Recently, the SNPs associated with fungicide resistance have been identified in genomes of plant pathogenic fungi .The occurrence rate for fungicide-resistant isolates is evaluated by estimating the SNP allele frequency.

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sequence‐controlled multiblock copolymers via raft

Sequence‐Controlled Multiblock Copolymers via RAFT

Multiblock copolymers represent a class of polymers with controlled monomer sequence distribution, where each individual block can vary significantly in degree of polymerization. 14 Such macromolecular structures have traditionally been limited to relatively few blocks, 15-18 but recent developments have enabled synthesis of highly complex high

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“raft” formation by two‐dimensional self‐assembly of block

“Raft” Formation by Two‐Dimensional Self‐Assembly of Block

Block copolymers can form a broad range of self‐assembled aggregates. In solution, planar assemblies usually form closed structures such as vesicles; thus, free‐standing sheet formation can be challenging. While most polymer single crystals are planar, their growth usually occurs by uptake of individual chains.

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complex polymer architectures via raft polymerization

Complex polymer architectures via RAFT polymerization

Reversible addition fragmentation chain transfer (RAFT) polymerization has made a huge impact in macromolecular design. The first block copolymers were described early on, followed by star polymers and then graft polymers. In the last five years, the types of architectures available have become more and more complex.

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raft-dna polymers - nano news

RAFT-DNA Polymers - Nano News

As a proof of concept we have been looking at conjugating single stranded oligonucleotides to a RAFT agent and then polymerising a hydrophilic monomer with the RAFT-Oligonucleotide agent. Many different methods of characterising these polymers are used to identify these new materials, one of which is polyacrylamide gel electrophoresis (PAGE).

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synthesis and properties of heterografted toothbrushlike

Synthesis and properties of heterografted toothbrushlike

terminal quaternized comblike block with tunable grafting density, middle block with alternating PEG and PCL grafts, and 50 terminal D segment as the handle. The resultant copolymers were characterized by 1H NMR spectra, GPC-MALLS, and DSC. In addition, the surface wettability of copolymer films and drug

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link to vor: angewandte angew. chem. int. ed. chemie

Link to VoR: Angewandte Angew. Chem. Int. Ed. Chemie

copolymers of the type ssDNA-b-A-b-B, with A and B denoting different synthetic polymer units, by the grafting-from approach. PISA by using RAFT polymerization has emerged prominently for the preparation of intricate block copolymer assemblies.[18,19] With increasing degrees of polymerization, the structures

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synthesis of dna conjugate by mechanochemical solid-state

Synthesis of DNA conjugate by mechanochemical solid-state

RAFT-generated polyacrylamide-DNA block copolymers for single-nucleotide polymorphism genotyping by affinity capillary electrophoresis. Kanayama N, Shibata H, Kimura A, Miyamoto D, Takarada T, Maeda M. Biomacromolecules, 10(4):805-813, 01 Apr 2009 Cited by: 6 articles | PMID: 19249847

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dna–polymer micelles as nanoparticles with recognition

DNA–Polymer Micelles as Nanoparticles with Recognition

The Watson–Crick binding of DNA single strands is a powerful tool for the assembly of nanostructures. Our objective is to develop polymer nanoparticles equipped with DNA strands for surface-patterning applications, taking advantage of the DNA technology, in particular, recognition and reversibility.

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neuron-targeted copolymers with sheddable shielding blocks

Neuron-targeted copolymers with sheddable shielding blocks

Polycations are an attractive class of material for gene delivery because they self-assemble with and condense nucleic acids, can be synthesized at large scale, and offer flexible chemistries for functionalization. 1 In the past few decades, many polymer compositions and architectures have been synthesized. In vitro screening of these polymers has yielded many materials that efficiently

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naoki kanayama(金山 直樹) - google scholar citations

Naoki Kanayama(金山 直樹) - Google Scholar Citations

RAFT-Generated Polyacrylamide-DNA Block Copolymers for Single-Nucleotide Polymorphism Genotyping by Affinity Capillary Electrophoresis N Kanayama, H Shibata, A Kimura, D Miyamoto, T Takarada, M Maeda Biomacromolecules 10 (4), 805-813 , 2009

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polystyrene-block-poly(acrylic acid), ddmat terminated ps

Polystyrene-block-poly(acrylic acid), DDMAT terminated PS

Application Polystyrene-block-polyacr ylic acid is a diblock copolymer commonly used for making polymeric vesicles (polymersomes) and other encapsulation applications.; This was generated through RAFT polymerization;and contains end-group functionalization that can be used to generate a triblock copolymer or modified for ligation. The 2-(Dodecylthiocarbonothio ylthio)-2-methylpropionic acid

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dna‐templated synthesis in three dimensions: introducing a

DNA‐Templated Synthesis in Three Dimensions: Introducing a

Blocked up : Amphiphilic DNA‐block copolymers were prepared in a fully automated single process by using a DNA synthesizer. These materials formed micelles in aqueous solution which were applied as a scaffold for DNA‐templated organic reactions (see picture, red and green balls=reactants, yellow bar=new bond).

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university of groningen dna multiblock copolymers

University of Groningen DNA multiblock copolymers

DNA multiblock copolymers{Fikri E. Alemdaroglu, Meryem Safak, Jie Wang, Ru¨diger Berger and Andreas Herrmann* Received (in Cambridge, UK) 20th October 2006, Accepted 2nd January 2007 First published as an Advance Article on the web 17th January 2007 DOI: 10.1039/b615276g Single stranded (ss) DNA block copolymers were applied to

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synthesis of dna block copolymers with extended nucleic

Synthesis of DNA block copolymers with extended nucleic

control, non-polymer-modified DNA of 795 bp with the same sequence as the block copolymers was employed. The DNA di- and triblock copolymer and the dsDNA were digested by the enzyme DraI at 37 °C for 3 h in the reaction buffer containing 33 mM Tris-acetate (pH 7.9), 10 mM magnesium acetate, 66 mM potassium acetate and 0.1 mg/ml BSA.

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optimization of brush-like cationic copolymers for non

Optimization of Brush-like Cationic Copolymers for Non

Decoration of P(GMA) homopolymer and block copolymer by oligoamines. P(GMA) homopolymers with DP of 50 and 100 were decorated by either TEPA, PEHA, or TREN oligoamines. Block copolymers of P(HPMA)/P(OEGMA)-b-P(GMA) 50 were decorated by the optimized oligoamine TEPA in a 30-fold molar excess. In a typical procedure, TEPA (2.0 mL, 10.5mmol)was

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biological–synthetic hybrid block copolymers: combining

Biological–synthetic hybrid block copolymers: Combining

Hideharu Mori, Motonobu Matsuyama and Takeshi Endo, Double‐Hydrophilic and Amphiphilic Block Copolymers Synthesized by RAFT Polymerization of Monomers Carrying Chiral Amino Acids, Macromolecular Chemistry and Physics, 210, 3‐4, (217-229), (2009).

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