electrophoresis | alfa aesar

Electrophoresis | Alfa Aesar

Gel electrophoresis is used to separate biological macromolecules. When an electric current is applied to these molecules in a gel matrix they can be separated based on their sizes and net charges. Proteins are typically separated with polyacrylamide as the gel matrix. Separation is affected by the percentage of acrylamide in the gel.

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electrophoresis reagents - alfa aesar

Electrophoresis Reagents - Alfa Aesar

Gel electrophoresis is used to separate biological macromolecules. When an electric current is applied to these molecules in a gel matrix they can be separated based on their sizes and net charges. Proteins are typically separated with polyacrylamide as the gel matrix. Separation is affected by the percentage of acrylamide in the gel matrix.

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electrophoresis - alfa.com

Electrophoresis - alfa.com

View and purchase reagents including sample and loading buffers, stains, and molecular weight markers available from Alfa Aesar. View more cellular and molecular products online at Alfa Aesar.

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dna/rna electrophoresis - alfa aesar

DNA/RNA Electrophoresis - Alfa Aesar

Gel electrophoresis separates fragments of nucleic acid that differ in size, charge or conformation. When charged molecules are placed in an electric field, they migrate toward either the positive or negative pole according to their charge. In contrast to proteins, which can have either a net positive or net negative charge, nucleic acids have a consistent negative charge imparted by their

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alfa aesar agarose, electrophoresis grade | fisher scientific

Alfa Aesar Agarose, Electrophoresis Grade | Fisher Scientific

Alfa Aesar Agarose, Electrophoresis Grade 50g Electrophoresis, Western Blotting and ELISA:Electrophoresis Good gel strength and clarity. Agarose is useful in separation of nucleic acids electrophoretically because agarose gels have larger pore sizes than cross linked acrylamide gels at low concentrations and in chromatographic separations

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electrophoresis - alfa.com

Electrophoresis - alfa.com

View and purchase reagents including sample and loading buffers, stains, and molecular weight markers available from Alfa Aesar. View more cellular and molecular products online at Alfa Aesar.

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alfa aesar

Alfa Aesar

Alfa Aesar is a leading manufacturer and supplier of research chemicals, pure metals and materials for a wide span of applications. Cookies disclaimer I agree Our site saves small pieces of text information (cookies) on your device in order to deliver better content and for statistical purposes.

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protein research | alfa aesar

Protein Research | Alfa Aesar

Recently, proteomics, the large scale study of proteins and their structures and functions, has emerged as a key area of research interest. The common work flow in proteomics research is extraction of proteins from biological samples, separation by gel electrophoresis, protein digestion, followed by mass spectrometry analysis.

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evaluation of fibroblasts adhesion and proliferation

Evaluation of Fibroblasts Adhesion and Proliferation

Protein patterns of gelatin released from the ADA-GEL films of different compositions after 7 days of incubation in serum free DMEM were analyzed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). SDS-PAGE was carried out using the Mini-PROTEAN 3 Cell system (Bio-Rad, Germany).

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supporting information electrochemiluminescent emitter

Supporting Information Electrochemiluminescent Emitter

1.4 Polyacrylamide gel electrophoresis (PAGE) analysis. The feasibility of target-induced dual-legged DNA-walker cycle amplification strategy was analyzed by loading specimens into the lanes with new-made 16% non-denatured polyacrylamide, and electrophoresis carried out at the potential of 100 V in 1 × TBE buffer for 1.5 h.

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purification of polyphenol oxidase from borage

Purification of polyphenol oxidase from borage

Polyacrylamide gel electrophoresis (PAGE) of the enzyme was performed according to the method of Laemmli (1970). Polyacrylamide gel (12%) was prepared for native and sodium dodecyl sulphate gel electrophoresis. After running, gel was dyed with Coomassie Brilliant Blue R-250 for SDS-PAGE and incubated in 30 mM 4-methylcatechol solution for 1 h

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purification and characterization of nitrilase

Purification and characterization of nitrilase

from Alfa Aesar (Germany), Sigma–Aldrich (USA) or Merck (Germany). Chemicals for protein sequencing were purchased from Applied BioSystems Inc. (USA). 2.2. Microorganisms and cultivation SDS polyacrylamide gel electrophoresis was performed according to Laemmli [16] in 12% polyacrylamide slab gels with protein molecular weight standards in

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correlating the serum albumin corona of zinc oxide

CORRELATING THE SERUM ALBUMIN CORONA OF ZINC OXIDE

was investigated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and by Fourier transform infrared spectroscopy (FTIR). 2. METHODS 2.1. NANOPARTICLES CHARACTERIZATION ZnO powder (noted as ZnO NPs 7–13 nm) was purchased by Alfa Aesar (TermoFischer, Kandel, Germany), and contains NPs of 7–13 nm size with a purity > 99%.

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supporting information dna-encircled lipid bilayers lars

Supporting Information DNA-Encircled Lipid Bilayers Lars

(8.3 nmol, 21 nt) were reacted with 245 nmol alkyl-iodide in 90% DMF (Alfa Aesar) and 10% 30 mM Tris-HCl pH 8.0 (1250 µL). The mixture was incubated at 65 °C for 3 h. The excess of the organic solvent was removed by gel permeation using NAP-25 columns (GE healthcare).

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altered characteristics of silica nanoparticles in bovine

Altered characteristics of silica nanoparticles in bovine

Many toxicological studies on silica nanoparticles (NPs) have been reported, however, the literature often shows various conclusions concerning the same material. This is mainly due to a lack of sufficient NPs characterization as synthesized as well as in operando. Many characteristics of NPs may be affected by the chemistry of their surroundings and the presence of inorganic and biological

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inhibition of glioblastoma cell proliferation, invasion

Inhibition of glioblastoma cell proliferation, invasion

Glioblastoma multiforme is one of the most aggressive brain tumors and current therapies with temozolomide or suberoylanilide hydroxamic acid (SAHA, vorinostat) show considerable limitations. SAHA

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electrophoresis reagents market by product & application

Electrophoresis Reagents Market by Product & Application

[224 Pages Report] The global electrophoresis reagents market is projected to reach USD 1.05 billion by 2020, at a CAGR of 5.4% from 2015 to 2020.Substantial growth and rapid technological advancement was experienced by the electrophoresis market in the past few years which had had a positive impact on the overall growth of the electrophoresis reagents market.

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selective binding of matrix metalloproteases mmp-9 and mmp

Selective binding of matrix metalloproteases MMP-9 and MMP

Selective binding of matrix metalloproteases MMP-9 and MMP-12 to inhibitor-assisted thermolysin-imprinted beads Nicole Schauer,†a Mehmet Dinc,†b Bastian Raabe,a Tim Hummel,a Marlen Muller,¨ a Harald Sobeka and Boris Mizaikoff *b Protein-imprinted polymers have been synthesized to recognize and specifically bind selected proteins.

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altered characteristics of silica nanoparticles in bovine

Altered characteristics of silica nanoparticles in bovine

Interactions of the particles with biological media was investigated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) in FBS and human serum, and extracted proteins were assessed using matrix-assisted laser desorption/ionization-time of flight technique (MALDI-TOF). (APTES, 98%, Alfa Aesar, Germany) and 3

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purification and characterization of nitrilase

Purification and characterization of nitrilase

from Alfa Aesar (Germany), Sigma–Aldrich (USA) or Merck (Germany). Chemicals for protein sequencing were purchased from Applied BioSystems Inc. (USA). 2.2. Microorganisms and cultivation SDS polyacrylamide gel electrophoresis was performed according to Laemmli [16] in 12% polyacrylamide slab gels with protein molecular weight standards in

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combinatorial discovery of cosolvent systems

Combinatorial Discovery of Cosolvent Systems

Combinatorial Discovery of Cosolvent Systems for Production of Narrow Dispersion Thiolate-Protected Gold Nanoparticles

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a quantitative model of thermal stabilization

A Quantitative Model of Thermal Stabilization

A Quantitative Model of Thermal Stabilization and Destabilization of Proteins by Ligands Piotras Cimmperman,* Lina Baranauskiene,* Simona Jachimovic_ ˇiute,_ y Jelena Jachno,* Jolanta Torresan,* Vilma Michailoviene,* Jurgita Matulien_e,* Jolanta Sereikaite,_ y Vladas Bumelis,y and Daumantas Matulis* *Laboratory of Biothermodynamics and Drug Design, Institute of Biotechnology, LT-02241 Vilnius

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frontiers in nanoscience and nanotechnology

Frontiers in Nanoscience and Nanotechnology

(anatase) particles were obtained from Alfa Aesar (Alfa Aesar GmbH & Co KG, Germany). Particles were used with a nominal size of 10 nm (Stock Number 44690, Lot B19T020; specific surface area 120 m2 g-1) and a measured size of 14.4 nm [13]. The particles form agglomerates in a size of 673 × 455 nm [13]. Stock solution was prepared with

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alpha- l -fucosidase isoenzyme iso2 from paenibacillus

Alpha- l -Fucosidase Isoenzyme iso2 from Paenibacillus

α-l-Fucosidases are enzymes involved in metabolism of α-l-fucosylated molecules, compounds with a fundamental role in different life essential processes including immune response, fertilization and development, but also in some serious pathological events. According to the CAZy database, these enzymes belong to families 29 and 95. Some of them are also reported to be able to catalyze

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selective binding of matrix metalloproteases mmp-9 and mmp

Selective binding of matrix metalloproteases MMP-9 and MMP

Selective binding of matrix metalloproteases MMP-9 and MMP-12 to inhibitor-assisted thermolysin-imprinted beads Nicole Schauer,†a Mehmet Dinc,†b Bastian Raabe,a Tim Hummel,a Marlen Muller,¨ a Harald Sobeka and Boris Mizaikoff *b Protein-imprinted polymers have been synthesized to recognize and specifically bind selected proteins.

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stabilization of immobilized lipases by intense

Stabilization of Immobilized Lipases by Intense

Immobilized enzymes have a very large region that is not in contact with the support surface and this region could be the target of new stabilization strategies. The chemical amination of these regions plus further cross-linking with aldehyde-dextran polymers is proposed here as a strategy to increase the stability of immobilized enzymes.

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design, synthesis and dna interactions of a chimera

Design, synthesis and DNA interactions of a chimera

Design, synthesis and DNA interactions of a chimera between a platinum complex and an IHF mimicking peptide†. Harita Rao a, Mariana S. Damian ab, Alak Alshiekh b, Sofi K. C. Elmroth b and Ulf Diederichsen * a a Institut für Organische und Biomolekulare Chemie, Georg-August-Universität Göttingen, Tammannstrasse 2, 37077 Göttingen, Germany.

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combinatorial discovery of cosolvent systems

Combinatorial Discovery of Cosolvent Systems

Combinatorial Discovery of Cosolvent Systems for Production of Narrow Dispersion Thiolate-Protected Gold Nanoparticles

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cdna cloning and characterization of vanadium-dependent

cDNA cloning and characterization of vanadium-dependent

cDNA cloning and characterization of vanadium-dependent bromoperoxidases from the red alga Laurencia nipponica Kensuke Kaneko, Kenji Washio, Taiki Umezawa, Fuyuhiko Matsuda, Masaaki Morikawa and Tatsufumi Okino* Graduate School of Environmental Science, Hokkaido University, Sapporo, Japan Received November 15, 2013; accepted March 3, 2014

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frontiers in nanoscience and nanotechnology

Frontiers in Nanoscience and Nanotechnology

(anatase) particles were obtained from Alfa Aesar (Alfa Aesar GmbH & Co KG, Germany). Particles were used with a nominal size of 10 nm (Stock Number 44690, Lot B19T020; specific surface area 120 m2 g-1) and a measured size of 14.4 nm [13]. The particles form agglomerates in a size of 673 × 455 nm [13]. Stock solution was prepared with

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activated carbon-carbon double bonds reduction

Activated Carbon-Carbon Double Bonds reduction

All reagents were purchased from Sigma-Aldrich or Alfa Aesar and were of the highest available purity. 3.2 Enzyme Preparations Genes coding for Johnson Matthey ENEs (ENE-101 TM , ENE-102 TM and ENE-103 TM ) were ordered codon-optimised from GeneArt ® (Thermo Fisher Scientific Inc) and cloned into T5 vector pJEx401 (DNA2.0).

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engineering of tm1459 from thermotoga maritima

Engineering of TM1459 from Thermotoga maritima

CA, USA). For sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), NuPAGE R 4–12% Bis-Tris Gels, 1.0 mm (Life Technologies, Carlsbad, CA, USA) were used with NuPAGE MES SDS Running Buffer. Cloning and Mutagenesis Site-saturation libraries of active site amino acids (Arg39, Phe41, Ile49, Trp56, Ile60, Phe94, Phe104, Cys106, and

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