cross-linked polyacrylamide coating for capillary

Cross-Linked Polyacrylamide Coating for Capillary

Polyacrylamide has been used for capillary wall coating for decades. The coating chemistry includes two main steps: (i) attachment of a bifunctional reagent containing a vinyl group to the silica surface and (ii) extension of the anchored vinyl groups through acrylamide polymerization. Since the introduction of this method, many modifications and improvements have been made.

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cross-linked polyacrylamide coating for capillary

Cross-Linked Polyacrylamide Coating for Capillary

Cross-Linked Polyacrylamide Coating for Capillary Isoelectric Focusing Article in Analytical Chemistry 76(24):7179-86 · January 2005 with 26 Reads How we measure 'reads'

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high efficiency cross‐linked polyacrylamide coating

High efficiency cross‐linked polyacrylamide coating

A highly efficient cross‐linked polyacrylamide capillary coating has been developed for capillary electrophoresis of proteins. The fused silica capillary tubing was pretreated with 7‐oct‐1‐enyltrimet...

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cross-linked polymer coatings for capillary

Cross-Linked Polymer Coatings for Capillary

A coating process for covalent coupling of fully formed polymers to silane-treated capillaries is described. The coupling process occurs through free-radical sites created on both the silane and polymer during the polymer cross-linking process. Coupling and cross-linking take place simultaneously, resulting in a densely cross-linked layer on the capillary surface.

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high efficiency cross‐linked polyacrylamide coating

High efficiency cross‐linked polyacrylamide coating

The full text of this article hosted at iucr.org is unavailable due to technical difficulties.

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replaceable cross-linked polyacrylamide for high

Replaceable Cross-Linked Polyacrylamide for High

An alternative sieving matrix, replaceable cross-linked polyacrylamide (rCPA), was developed for sodium dodecyl sulfate capillary gel electrophoresis (SDS-CGE) separation of proteins. This rCPA could be conveniently pressurized into separation capillaries under a pressure of 80 psi. SDS-CGE separations using this matrix generated high resolutions for a wide range (∼4 kD to ∼300 kD) of

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a robust cross-linked polyacrylamide coating for microchip

A robust cross-linked polyacrylamide coating for microchip

A robust cross-linked polyacrylamide coating for microchip electrophoresis of dsDNA fragments Article in Electrophoresis 27(19):3764-71 · October 2006 with 11 Reads How we measure 'reads'

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replaceable cross-linked polyacrylamide for high

Replaceable Cross-Linked Polyacrylamide for High

An alternative sieving matrix, replaceable cross-linked polyacrylamide (rCPA), was developed for sodium dodecyl sulfate capillary gel electrophoresis (SDS-CGE) separation of proteins. This rCPA could be conveniently pressurized into separation capillaries under a pressure of 80 psi. SDS-CGE separations using this matrix generated high resolutions for a wide range (∼4 kD to ∼300 kD) of

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capillary electrophoresis and isoelectric focusing

Capillary electrophoresis and isoelectric focusing

3 Capillary isoelectric focusing. CIEF, in presence of soluble carrier ampholytes (CAs), is a method of reasonable success in protein separation and analysis. The capillary has to be coated, of course, better if with a covalently affixed polymer, such as polyacrylamide or other suitable, hydrophilic coating.

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de19938002a1 - coating with cross-linked hydrophilic

DE19938002A1 - Coating with cross-linked hydrophilic

DE19938002A1 DE1999138002 DE19938002A DE19938002A1 DE 19938002 A1 DE19938002 A1 DE 19938002A1 DE 1999138002 DE1999138002 DE 1999138002 DE 19938002 A DE19938002 A DE 19938002A DE 19938002 A1 DE19938002 A1 DE 19938002A1 Authority DE Germany Prior art keywords capillary capillaries method according polymer cross Prior art date 1999-08-11 Legal status (The legal status is an assumption and is not

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gel electrophoresis of proteins - gbv

Gel Electrophoresis of Proteins - GBV

2. Fundamentals of capillary gel electrophoresis 106 3. Operational variables 108 Applied electric field and temperature 109 Capillary dimensions 109 Capillary coating 110 Gels and polymer solutions 112 4. Separations 113 Cross-linked polyacrylamide gel 113 Linear polyacrylamide solutions 115 Other entangled polymer solutions 116 The Ferguson

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photopolymerized cross-linked polyacrylamide gels for on

Photopolymerized Cross-Linked Polyacrylamide Gels for On

Photopolymerized Cross-Linked Polyacrylamide Gels for On-Chip Protein Sizing Amy E. Herr* and Anup K. Singh Biosystems Research Department, Sandia National Laboratories, Livermore, California 94551 A new method for on-chip sodium dodecyl sulfate poly-acrylamide gel electrophoresis (SDS-PAGE) of proteins is reported.

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the advances of the methods for preparation of the coated

The Advances of the Methods for Preparation of the Coated

The most successful method for controlling the adsorption of proteins onto the capillary inner surface in CE is the modification of the capillary surface with coating. The advances of the method for preparation of the coated capillaries are systematically reviewed with

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capillary electrophoresis - gbv

capillary electrophoresis - GBV

6.7.3 Coatings for capillary isoelectric focusing 344 6.7.4 Buffer or sample additives in capillary isoelectric focusing 345 6.7.5 Detection for capillary isoelectric focusing 347 6.8 Capillary isotachophoresis 347 6.8.1 Theory of capillary isotachophoresis (CITP) 348 6.8.2 Capillary isotachophoresis with electroosmotic flow 352

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美国药典usp34中记载毛细管电泳的在哪一页(一次性发放

美国药典USP34中记载毛细管电泳的在哪一页(一次性发放

Optimization of separation in a cross-linked gel is obtained by modifying the separation buffer (see Capillary Zone Electrophoresis ) and by controlling the gel porosity during the gel preparation. For cross-linked polyacrylamide gels, the porosity can be modified by changing the concentration of acrylamide and/or the ratio of the cross-linker.

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<1053> biotechnology-derived articles-capillary

<1053> BIOTECHNOLOGY-DERIVED ARTICLES-CAPILLARY

This chapter provides guidance and procedures used for characterization of biotechnology-derived articles by capillary electrophoresis. This chapter is harmonized with the corresponding chapter in JP and EP.Other characterization tests, also harmonized, are shown in Biotechnology-Derived Articles—Amino Acid Analysis 1052, Biotechnology-Derived Articles—Isoelectric Focusing 1054

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photopolymerized cross-linked polyacrylamide gels for on

Photopolymerized Cross-Linked Polyacrylamide Gels for On

Photopolymerized Cross-Linked Polyacrylamide Gels for On-Chip Protein Sizing Amy E. Herr* and Anup K. Singh Biosystems Research Department, Sandia National Laboratories, Livermore, California 94551 A new method for on-chip sodium dodecyl sulfate poly-acrylamide gel electrophoresis (SDS-PAGE) of proteins is reported.

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journal of chromatography, 347 (1985) 191-198 elsevier

Journal of Chromatography, 347 (1985) 191-198 Elsevier

a mono-molecular layer of non-cross-linked polyacrylamide. The coating procedure is described in detail. INTRODUCTION Zone deformation caused by the Joule heat is often negligible in high-perform- ance electrophoresis, since the separations take place in thin-walled, narrow-bore (0.05-0.3 mm I.D.) tubes.

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publications - oucreate.com

Publications - oucreate.com

Joann J. Lu and Shaorong Liu, A Robust Cross-Linked Polyacrylamide Coating for Microchip Electrophoresis of dsDNA Fragments, Electrophoresis, 19 (2006), 3764-71. Shaorong Liu, Qiaosheng Pu, Lin Gao, and Joann Lu, An economic approach for construction of a multiplexed capillary electrophoresis system, Talanta, 70 (2006), 644-650.

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us6596238b1 - coatings with cross-linked hydrophilic

US6596238B1 - Coatings with cross-linked hydrophilic

The present invention relates to a process for permanently coating the inner surface of columns, capillaries and microchannel systems with hydroxylic polymers, such as polyvinyl alcohol, and to the thus prepared columns, capillaries and microchannel systems and their use. US6596238B1 - Coatings with cross-linked hydrophilic polymers

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capillary array isoelectric focusing with laser-induced

Capillary array isoelectric focusing with laser-induced

Capillary array isoelectric focusing with laser-induced fluorescence detection: milli-pH unit resolution 0.0008 pH. Capillary coating stability limits the reproduc- performed using a set of 32 cross-linked polyacrylamide coated capillaries, 30 cm long 50/150 µm I.D/O.D. The

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polymer wall coatings for capillary electrophoresis

Polymer wall coatings for capillary electrophoresis

Polymer wall coatings for capillary electrophoresis Polymer wall coatings for capillary electrophoresis Horvath, Judit; Dolník, Vladislav 2001-02-01 00:00:00 Judit Horvath Vladislav Dolník Molecular Dynamics Inc., Sunnyvale, CA, USA Polymer wall coatings for capillary electrophoresis This review article describes the preparation of dynamic and static polymeric wall coatings for capillary

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<1053> biotechnology-derived articles-capillary

<1053> BIOTECHNOLOGY-DERIVED ARTICLES-CAPILLARY

This chapter provides guidance and procedures used for characterization of biotechnology-derived articles by capillary electrophoresis. This chapter is harmonized with the corresponding chapter in JP and EP.Other characterization tests, also harmonized, are shown in Biotechnology-Derived Articles—Amino Acid Analysis 1052, Biotechnology-Derived Articles—Isoelectric Focusing 1054

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美国药典usp34中记载毛细管电泳的在哪一页(一次性发放

美国药典USP34中记载毛细管电泳的在哪一页(一次性发放

Optimization of separation in a cross-linked gel is obtained by modifying the separation buffer (see Capillary Zone Electrophoresis ) and by controlling the gel porosity during the gel preparation. For cross-linked polyacrylamide gels, the porosity can be modified by changing the concentration of acrylamide and/or the ratio of the cross-linker.

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photopolymerized cross-linked polyacrylamide gels for on

Photopolymerized Cross-Linked Polyacrylamide Gels for On

Photopolymerized Cross-Linked Polyacrylamide Gels for On-Chip Protein Sizing Amy E. Herr* and Anup K. Singh Biosystems Research Department, Sandia National Laboratories, Livermore, California 94551 A new method for on-chip sodium dodecyl sulfate poly-acrylamide gel electrophoresis (SDS-PAGE) of proteins is reported.

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us4542200a - polyacrylamide cross-linked with a

US4542200A - Polyacrylamide cross-linked with a

US4542200A US06/677,101 US67710184A US4542200A US 4542200 A US4542200 A US 4542200A US 67710184 A US67710184 A US 67710184A US 4542200 A US4542200 A US 4542200A Authority US United States Prior art keywords polyacrylamide gel cross linked acrylamide Prior art date 1982-02-19 Legal status (The legal status is an assumption and is not a legal conclusion.

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us patent for neutral polysaccharide wall coating

US Patent for Neutral polysaccharide wall coating

The present invention describes a method of preparation of a permanent neutral wall coating made of thermally immobilized polysaccharides. The coating suppresses electroosmotic flow and adsorption on the wall under acidic, neutral, and basic conditions in capillary electrophoresis.

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journal of separation science : vol 37 , no 21

Journal of Separation Science : Vol 37 , No 21

Furthermore, coating methods for the resistance of protein non‐selective adsorption and simultaneously for pH gradient photo‐coupling were screened. The poly (ethylene glycol)‐silane coating method was found to be better than the cross‐linked polyacrylamide coating and aminosilane modification methods.

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polymer wall coatings for capillary electrophoresis

Polymer wall coatings for capillary electrophoresis

Polymer wall coatings for capillary electrophoresis Polymer wall coatings for capillary electrophoresis Horvath, Judit; Dolník, Vladislav 2001-02-01 00:00:00 Judit Horvath Vladislav Dolník Molecular Dynamics Inc., Sunnyvale, CA, USA Polymer wall coatings for capillary electrophoresis This review article describes the preparation of dynamic and static polymeric wall coatings for capillary

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us patent for coatings with cross-linked hydrophilic

US Patent for Coatings with cross-linked hydrophilic

Coatings with cross-linked hydrophilic polymers Aug 9, 2000 - Studiengesellschaft Kohle mbH The present invention relates to a process for permanently coating the inner surface of columns, capillaries and microchannel systems with hydroxylic polymers, such as polyvinyl alcohol, and to the thus prepared columns, capillaries and microchannel

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covalently cross-linked, mixed-bed agarose-polyacrylamide

Covalently cross-linked, mixed-bed agarose-polyacrylamide

Covalently cross-linked, mixed-bed agarose-polyacrylamide matrices for electrophoresis and chromatography are disclosed. The mixed-bed matrices are formed by the copolymerization of a substituted agarose mixture with acrylamide monomers.

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美国药典usp34中记载毛细管电泳的在哪一页(一次性发放

美国药典USP34中记载毛细管电泳的在哪一页(一次性发放

Optimization of separation in a cross-linked gel is obtained by modifying the separation buffer (see Capillary Zone Electrophoresis ) and by controlling the gel porosity during the gel preparation. For cross-linked polyacrylamide gels, the porosity can be modified by changing the concentration of acrylamide and/or the ratio of the cross-linker.

Get Price